Characterization of polyphenol oxidase from Chaetomium thermophile, a thermophilic fungus.

PARTIAL PURIFICATION AND CHARACTERIZATION OF POLYPHENOL OXIDASE FROM THERMOPHILIC Bacillus sp

Direct Electrochemistry of Polyphenol Oxidase

The electrochemistry of banana tissues on a carbon paste electrode modified with multi-walled carbon nanotubes (MWCNTs) is presented. Cyclic voltammetry is applied to investigate the direct electrochemistry of banana tissues i.e. a source of polyphenol oxidase (PPO). A redox couple with an anodic and counterpart cathodic peak is obtained. The influence of various parameters such as pH,...

Purification and characterization of polyphenol oxidase from fresh ginseng

Polyphenol oxidase (PPO) was purified from fresh ginseng roots using acetone precipitation, carboxymethyl (CM)-Sepharose chromatography, and phenyl-Sepharose chromatography. Two isoenzymes (PPO 1 and PPO 2) were separated using an ion-exchange column with CM-Sepharose. PPO 1 was purified up to 13.2-fold with a 22.6% yield. PPO 2 bound to CM-Sepharose, eluted with NaCl, and was purified up to 22...

Transcriptional Profiling of ESTs from the Biocontrol Fungus Chaetomium cupreum

Comparative analysis was applied to two cDNA/ESTs libraries (C1 and C2) from Chaetomium cupreum. A total of 5538 ESTs were sequenced and assembled into 2162 unigenes including 585 contigs and 1577 singletons. BlastX analysis enabled the identification of 1211 unigenes with similarities to sequences in the public databases. MFS monosaccharide transporter was found as the gene expressed at the hi...

Cloning and Characterization of Polyphenol Oxidase cDNAs of Phyfolacca americana'

Two cDNA clones encoding polyphenol oxidases were isolated from a cDNA library constructed from a log-phase suspension culture of Phyfolacca americana (pokeweed) producing betalains. The clones exhibit 93 and 86% sequence identity at the nucleotide and deduced amino acid levels, respectively. Both clones contain two copper-binding domains characterized by histidine-rich regions, which are found...